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Image Search Results
Journal: Cells
Article Title: Methylglyoxal Impairs the Pro-Angiogenic Ability of Mouse Adipose-Derived Stem Cells (mADSCs) via a Senescence-Associated Mechanism
doi: 10.3390/cells12131741
Figure Lengend Snippet: mADSCs characterization. ( a ) Representative photographs (5× magnification of an optical microscope, scale bar 500 µm) of mADSCs isolated from the subcutaneous adipose tissue of C57bl6 mice cultured in selection medium for 4 passages. ( b ) Accumulated cell number and ( c ) population doubling time (PTD) per hours of mADSCs from p3 to p11. Once 90% confluence was reached, cells were counted by an automated cell counter. Dots in line graphs represent the mean ± SEM values. ( d – i ) Representative histograms of the expression of CD44, CD29, CD90.2, Sca-1, CD45 and CD31 surface markers analyzed by FACS.
Article Snippet:
Techniques: Microscopy, Isolation, Cell Culture, Selection, Expressing
Journal: PLoS ONE
Article Title: Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation
doi: 10.1371/journal.pone.0038917
Figure Lengend Snippet: Mice were challenged with LPS via inhalation and sacrificed 4 hours later. In addition, neutrophils were depleted by antibody injection or mice were treated with simvastatin (2 µg/g bodyweight) 12 hours and one hour before or one hour after LPS exposure as indicated. A: Quantification of alveolar (top), interstitial (middle), and intravascular neutrophils (bottom) in mice treated as indicated. B: FITC-dextran clearance (top), albumin concentration (middle), and elastase activity (bottom) in BAL fluids in mice treated as indicated (n = 8–10 for each bar). Statistical significance was tested using one way ANOVA with Newman-Keuls Multiple Comparison test. * indicates significant difference compared to LPS-treated animals.
Article Snippet: Cell pellets were labeled with PerCP-Cy5.5 anti-mouse Ly-6G, PE anti-mouse CD115, APC-Cy7 anti-mouse CD45 und APC anti-Mouse Ly- 6G (all eBioscience) and
Techniques: Injection, Concentration Assay, Activity Assay, Comparison
Journal: PLoS ONE
Article Title: Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation
doi: 10.1371/journal.pone.0038917
Figure Lengend Snippet: Isolated human neutrophils were pre-treated with Simvastatin (3 hours, 1 or 10 µM) and then activated with fMLP. MFI of surface expression of CD11b (A), CD29 (B) and FPRL1 (C) as measured by flow cytometry after staining with directly conjugated antibodies (n = 3–6; repetition = 4). D+E In vivo degranulation after LPS inhalation. Mice were challenged with LPS via inhalation and sacrificed 4 hours later. After isolation of neutrophils from the blood and the lung, MFI of surface expression of CD11b and CD29 as measured by flow cytometry after staining with directly conjugated antibodies (n = 4–6). Statistical significance was tested using one way ANOVA with Newman-Keuls Multiple Comparison test. * indicates significant difference in comparison to the fMLP or the LPS group.
Article Snippet: Cell pellets were labeled with PerCP-Cy5.5 anti-mouse Ly-6G, PE anti-mouse CD115, APC-Cy7 anti-mouse CD45 und APC anti-Mouse Ly- 6G (all eBioscience) and
Techniques: Isolation, Expressing, Flow Cytometry, Staining, In Vivo, Comparison
Journal: Biomedical Reports
Article Title: Comparative study of biological characteristics of mesenchymal stem cells isolated from mouse bone marrow and peripheral blood
doi: 10.3892/br.2019.1236
Figure Lengend Snippet: Flow cytometry antibody panel used for characterization.
Article Snippet:
Techniques: Flow Cytometry
Journal: Biomedical Reports
Article Title: Comparative study of biological characteristics of mesenchymal stem cells isolated from mouse bone marrow and peripheral blood
doi: 10.3892/br.2019.1236
Figure Lengend Snippet: Flow cytometric analysis of BM-MSCs and PB-MSCs. BM-MSCs were positive CD29 and negative for other markers, while PB-MSCs were positive for CD146, CD29, and CD140b and negative for Sca-1, CD44, CD45, CD90 and CD105. CD, cluster of differentiation; BM-MSCs, bone marrow-mesenchymal stromal cells; PB, peripheral blood; FITC, fluorescein isothiocyanate; PE, phycoerythrin.
Article Snippet:
Techniques:
Journal: bioRxiv
Article Title: Evaluation of M2-like macrophage enrichment after diffuse traumatic brain injury through transient interleukin-4 expression from engineered mesenchymal stromal cells
doi: 10.1101/2020.01.28.918441
Figure Lengend Snippet: (A) MSCs (Cyagen) used in this study expressed CD29, CD44, and Sca-1 while remaining negative for CD31 and CD45 in at least two late passage numbers.
Article Snippet: For MSC cell-surface characterization, the following antibodies were used:
Techniques:
Journal: Clinical Medicine Insights. Arthritis and Musculoskeletal Disorders
Article Title: Analysis of Mitogen-Activated Protein Kinases in Bone and Cartilage of Patients with Rheumatoid Arthritis Treated with Abatacept
doi: 10.4137/CMAMD.S34424
Figure Lengend Snippet: Comparison of MAPK expression of bone marrow by abatacept.
Article Snippet: The experimental primary antibodies were described earlier, such as anti-TNF-α mouse monoclonal antibody (1:1,000 dilution; Biogenesis), antihuman IL-6 rabbit polyclonal antibody (1:500, Rockland), antihuman CD4, anti-human CD68 mouse monoclonal antibody (1:1,000; DAKO), antihuman OPN mouse monoclonal antibody (1:250; Novo-castra), antihuman OPG, rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology), antihuman RANKL (FL-317) rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology),
Techniques: Expressing
Journal: Clinical Medicine Insights. Arthritis and Musculoskeletal Disorders
Article Title: Analysis of Mitogen-Activated Protein Kinases in Bone and Cartilage of Patients with Rheumatoid Arthritis Treated with Abatacept
doi: 10.4137/CMAMD.S34424
Figure Lengend Snippet: Comparison of MAPK expression of cartilage by abatacept.
Article Snippet: The experimental primary antibodies were described earlier, such as anti-TNF-α mouse monoclonal antibody (1:1,000 dilution; Biogenesis), antihuman IL-6 rabbit polyclonal antibody (1:500, Rockland), antihuman CD4, anti-human CD68 mouse monoclonal antibody (1:1,000; DAKO), antihuman OPN mouse monoclonal antibody (1:250; Novo-castra), antihuman OPG, rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology), antihuman RANKL (FL-317) rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology),
Techniques: Expressing
Journal: Clinical Medicine Insights. Arthritis and Musculoskeletal Disorders
Article Title: Analysis of Mitogen-Activated Protein Kinases in Bone and Cartilage of Patients with Rheumatoid Arthritis Treated with Abatacept
doi: 10.4137/CMAMD.S34424
Figure Lengend Snippet: Immunohistochemical comparison of the expression of MAPK in bone (magnification, 200×; black arrow shows strong positive). ( A ) and ( B ): CD29 (β-1 integrin); ( C ) and ( D ): JNK; ( E ) and ( F ): ERK; ( G ) and ( H ): P38 MAPK. ( A ), ( C ), ( E ), and ( G ): control group (MTX); ( B ), ( D ), ( F ), and ( H ): abatacept group.
Article Snippet: The experimental primary antibodies were described earlier, such as anti-TNF-α mouse monoclonal antibody (1:1,000 dilution; Biogenesis), antihuman IL-6 rabbit polyclonal antibody (1:500, Rockland), antihuman CD4, anti-human CD68 mouse monoclonal antibody (1:1,000; DAKO), antihuman OPN mouse monoclonal antibody (1:250; Novo-castra), antihuman OPG, rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology), antihuman RANKL (FL-317) rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology),
Techniques: Immunohistochemical staining, Expressing
Journal: Clinical Medicine Insights. Arthritis and Musculoskeletal Disorders
Article Title: Analysis of Mitogen-Activated Protein Kinases in Bone and Cartilage of Patients with Rheumatoid Arthritis Treated with Abatacept
doi: 10.4137/CMAMD.S34424
Figure Lengend Snippet: Immunohistochemical comparison of the expression of MAPK in cartilage (magnification, 200×; black arrow shows strong positive). ( A ) and ( B ): CD29 (β-1 integrin); ( C ) and ( D ): JNK; ( E ) and ( F ): ERK; ( G ) and ( H ): P38 MAPK. ( A ), ( C ), ( E ), and ( G ): control group (MTX); ( B ), ( D ), ( F ), and ( H ): abatacept group.
Article Snippet: The experimental primary antibodies were described earlier, such as anti-TNF-α mouse monoclonal antibody (1:1,000 dilution; Biogenesis), antihuman IL-6 rabbit polyclonal antibody (1:500, Rockland), antihuman CD4, anti-human CD68 mouse monoclonal antibody (1:1,000; DAKO), antihuman OPN mouse monoclonal antibody (1:250; Novo-castra), antihuman OPG, rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology), antihuman RANKL (FL-317) rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology),
Techniques: Immunohistochemical staining, Expressing